• Analyze the transcriptional response of root hair cells under conditions of abiotic stress (i.e., heat and drought stress)
  • Analyze proteomic and metabolomic changes in root hair cells under conditions of abiotic stress (i.e., heat and drought stress)
  • Utilize the tools and information developed in Objectives 1-2 to develop descriptive models of root hair metabolic function.

I.

Analyze the transcriptional response of root hair cells to abiotic stress. We will utilize high-throughput (HTP) Illumina Solexa cDNA sequencing to identify specific root hair mRNAs and sRNAs whose expression responds to abiotic stress.  Heat stress will be applied by growing plants under conditions of increasing temperature (>30oC). Drought stress will be applied by including polyethylene glycol in the germination medium.The goal will be to define the global response of a single, differentiated cell type, the root hair, to abiotic stress conditions that are pertinent to crop production and global climate change. e

II.

Analyze proteomic and metabolomic changes in root hair cells under conditions of abiotic stress (i.e., heat and drought stress). The root hoair proteome and metabolome will be analyzed under conditions of heat and drought stress. This work will be conducted in conjunction with our collaborators at the Environmental Molecular Sciences Laboratory at Pacific Northwest National Laboratory.

III.

Develop and apply computational systems biology methods to study genes, proteins, non-coding RNAs, metabolites and pathways involved in the root response to abiotic stress. hair cell biology by integrating dissimilar experimental data. To complement the above studies, objective 3 will develop and apply computational systems biology methods to study genes, proteins, non-coding RNAs, metabolites and pathways involved in the root hair response to abiotic stress.


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