We utilized root hair infection by the symbiotic bacterium, Bradyrhizobium japonicum as a tool to perturb cellular function. For example, Libault et al. (2010) utilized Affymetrix DNA microarray hybridization, high-throughput Illumina Solexa cDNA sequencing, and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) to show that over 48,000 of the 69,000 predicted soybean ORFs were expressed in soybean root hairs. These expression data were used to improve the current soybean genome annotation; identifying new ORFs, defining splice variants, extending genes both 5’ and 3’, and providing transcriptional support for the annotated genes.
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