Research Progress
- Bioinformatic Tools for Gene Expression Analysis
- Root Hair Phosphoproteome
- DOE Project Objectives
- Abiotic Stress
- Soybean Knowledge Base
- HTP sequencing of small RNA from inoculated soybean root hairs
- Root hair metabolomics
- Soybean gene expression atlas
- Soybean transcription factors
- Root hair transcriptome
DNA Microarray Methods
RESEARCH
We mined the soybean genome and identified over 5500 putative transcription factors (Libault et al., 2009). A searchable database focused on the soybean TF genes was developed (http://casp.rnet.missouri.edu/soydb/) (Wang et al., 2009). We utilized both qRT-PCR and Illumina Solexa cDNA sequencing to identify 204 TF genes that responded directly to B. japonicum inoculation. We also showed that RNAi silencing of a specific Myb TF gene significantly reduced soybean nodulation (Libault et al., 2009).