Research Progress
- Bioinformatic Tools for Gene Expression Analysis
- Root Hair Phosphoproteome
- DOE Project Objectives
- Abiotic Stress
- Soybean Knowledge Base
- HTP sequencing of small RNA from inoculated soybean root hairs
- Root hair metabolomics
- Soybean gene expression atlas
- Soybean transcription factors
- Root hair transcriptome
DNA Microarray Methods
RESEARCH
Our manuscript (Brechenmacher et al., 2010) describes the polar and non-polar root hair and stripped root metabolites. Metabolites were analyzed after water and methanol/ chloroform extraction by GC-MS and after extraction with 80% methanol by UPLC-MS. A total of 1691 metabolites were identified by combining GC-MS and LC-MS approaches, with 134 responding significantly to inoculation (0-48 HAI). Principal component analysis clearly segregated root hair from stripped root metabolites. A genome browser was developed (http://soymetdb.org/) that allows easy access to these data. This website is also meant to serve as a depository and analysis tool for other soybean metabolomics data.